《植物生理学报》 2012, 48(7): 664-668

崖白菜的愈伤组织诱导及植株再生

杨精华¹, 刘曦¹, 张乐¹, 王玉兵¹, 杨敬元², 梁宏伟^1,*

1三峡大学生物技术研究中心, 湖北宜昌443002; 2神农架金丝猴保育生物学湖北省重点实验室, 湖北神农架442421

通信作者：梁宏伟；E-mail: lianghwcn@yahoo.com.cn；Tel: 0717-6397188

摘　要：

通过愈伤组织诱导不定芽发生途径, 建立了崖白菜的组培再生体系, 研究了崖白菜幼嫩子房和不同植物生长调节剂 对其愈伤组织诱导和不定芽发生的影响。结果表明, 以崖白菜的幼嫩子房作为外植体, 诱导子房分化形成愈伤组织的最适 培养基为MS+6-BA 1.0 mg•L-1+2,4-D 0.2 mg•L-1, 诱导率可达82.83%; 最适的不定芽分化培养基为MS+6-BA 0.5 mg•L-1+NAA

关键词：崖白菜; 愈伤组织; 不定芽; 植株再生

收稿：2012-03-05   修定：2012-04-29

资助：神农架金丝猴保育生物学湖北省重点实验室开发基金(2012AB002C)。

Callus Induction and Plant Regeneration of Rehmannia rupestris Hemsl

YANG Jing-Hua¹, LIU Xi¹, ZHANG Le¹, WANG Yu-Bing¹, YANG Jing-Yuan², LIANG Hong-Wei^1,*

¹Biotechnology Research Center, China Three Gorges University, Yichang, Hubei 443002, China; ²Hubei Provincial Key Laboratory of Conservation Biology of Golden Monkey in Shennongjia, Shennongjia, Hubei 442421, China

Corresponding author: LIANG Hong-Wei; E-mail: lianghwcn@yahoo.com.cn; Tel: 0717-6397188

Abstract:

Tissue culture regeneration system of Rehmannia rupestris was established by the way to induce adventitious shoots. Young ovary and different plant growth regulators were discussed for callus induction and adventitious buds differentiation. The results showed that young ovary of Rehmannia rupestris served as explants, the best medium for callus formation was MS+6-BA 1.0 mg·L^-1+2,4-D 0.2 mg·L^-1, induction rate reached 82.83%; the optimum adventitious buds differentiation medium was MS+6-BA 0.5 mg·L^-1+NAA 0.1 mg·L^-1; in addition, the medium of 1/2MS+NAA 0.1 mg·L^-1 had the best effect on root formation.

Key words: Rehmannia rupestris; callus; adventitious buds; plant regeneration